Primary Structure of Human Fibrinogen and Fibrin
نویسندگان
چکیده
منابع مشابه
Primary structure of human fibrinogen and fibrin. Isolation and partial characterization of chains of fragment D.
Fragment D has been isolated as an apparently single molecular weight species (molecular weight about 100,000) from plasmin digests of humman fibrinogen, using a combination of affinity chromatography on insolubilized "fibrin monomer" and gel filtration. This fragment consists of three chains with molecular weights of 15,000 (Dbeta), 42,500 (Dgamma1) or 39,500 (Dgamma2), and 14,000 (Dalpha) hel...
متن کاملSubunit structure of human fibrinogen, soluble fibrin, and cross-linked insoluble fibrin.
The three unique polypeptide chains of human fibrinogen differ significantly in molecular weight. Cross-linkage of fibrin by fibrin-stabilizing factor results in the rapid formation of cross-links between gamma-chains and a slower formation of cross-links between alpha-chains. beta-Chains are not involved directly in the cross-linking of fibrin. Reduced, cross-linked fibrin contains uncross-lin...
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The cleavage of human fibrinogen in 70 % formic acid with CNBr has been studied. A fragment containing the NH%terminal portions of the three chains has been obtained from the reaction mixture. After reduction and alkylation the NH%-terminal fragments of the a(A) and a(AP) chains have been isolated in essentially pure form by gel filtration and chromatography. The amino acid sequence of these fr...
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The binding of human plasminogen to fibrin was studied using 125Lplasminogen and uniform suspensions of noncross-linked or cross-linked human fibrin. Of the two types of plasminogen, the proteolytically modified form (NJ&-terminal lysine; Lys-plasminogen) showed a high binding affinity for both forms of fibrin, having a dissociation constant of 0.32 p~ and two binding sites per fibrin monomeric...
متن کاملFibrinogen-fibrin degradation products.
The identification of the molecular structure of fibrinogen has given a better understanding of many clinical conditions associated with abnormalities of fibrinogen. Proteolysis of fibrinogen or fibrin by its natural lytic enzyme, plasmin, leads to the formation of degradation products which are of great importance in the laboratory diagnosis of many pathological conditions. The biochemical and...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1972
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)45586-5